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S. cerevisiae Advanced Gateway Destination Vectors

Plasmid Kit

#1000000011

Purpose

Destination vectors for tagging and expression of genes in S. cerevisiae.

Depositor

Susan Lindquist

Application

Cloning and Synthetic Biology, Gene Expression and Labeling

Vector Type

Yeast Expression

Cloning Type

Gateway

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only
CRISPR-Cas9 Gateway System for Physcomitrella patens

Plasmid Kit

#1000000151

Purpose

System for genome editing of the moss Physcomitrella patens.

Depositor

Magdalena Bezanilla

Application

Genome Editing

Vector Type

Plant Expression

Editing Type

CRISPR

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

Plasmid Kit

#1000000087

Purpose

A plasmid collection, based on the Tol2kit, used to streamline the production of transgenic zebrafish for studying neurodegenerative disorders.

Depositor

Nicholas Cole

Application

Cloning and Synthetic Biology

Vector Type

Zebrafish Expression

Cloning Type

MultiSite Gateway

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only
pLEX_305

Plasmid

#41390

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

Depositor

David Root

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX_306

Plasmid

#41391

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

Depositor

David Root

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX_307

Plasmid

#41392

Purpose

Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDest-527

Plasmid

#11518

Purpose

Gateway destination vector for bacterial expression; His tag

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

6xHis

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pDest-565

Plasmid

#11520

Purpose

Gateway destination vector for bacterial expression; His,GST tag

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

6xHis and GST

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCW57.1

Plasmid

#41393

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLIX_403

Plasmid

#41395

Purpose

Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

V5

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDest-544

Plasmid

#11519

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

His6-NusA

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pLEX_305

Plasmid

#41390

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

Depositor

David Root

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX_306

Plasmid

#41391

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

Depositor

David Root

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb + mcherry

Plasmid

#233303

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

Ubi:ccdb + mcherry

Use

Gateway destination

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb + RUBY

Plasmid

#233306

Purpose

GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

Ubi:ccdb + RUBY

Use

Gateway destination

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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DEXsynth:ccdb+ RUBY

Plasmid

#233320

Purpose

GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

DEXsynth:ccdb+ RUBY

Use

Gateway destination

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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DEXsynth:ccdb+ mcherry

Plasmid

#233315

Purpose

GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

DEXsynth:ccdb+ mcherry

Use

Gateway destination

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb-eGFP + RUBY

Plasmid

#233310

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

Ubi:ccdb-eGFP + RUBY

Use

Gateway destination

Tags

eGFP

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb-3xHA + mcherry

Plasmid

#233302

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

Ubi:ccdb-3xHA + mcherry

Use

Gateway destination

Tags

3xHA

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb-eGFP + mcherry

Plasmid

#233300

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

Depositor

Thomas Baum

Insert

Ubi:ccdb-eGFP + mcherry

Use

Gateway destination

Tags

eGFP

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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