We narrowed to 707 results for: pET-15b

1. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b CnA CnB

   Plasmid

   #11787

   Depositor

   Anjana Rao

   Article

   Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

   Insert

   CnA CnB (PPP3R1, PPP3CA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pET-15b-PTS

   Plasmid

   #108953

   Purpose

   heterologous expression of PTS of P. cablin in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Patchoulol synthase (PTS)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet-15b-microID

   Plasmid

   #172881

   Purpose

   expresses microID in E. coli

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   microID
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. wt HIV-1 integrase in pET15b

   Plasmid

   #61668

   Purpose

   wild type HIV-1 integrase in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   wild type HIV-1 integrase
   

   Use

   Tags

   His

   Expression

   Mutation

   Promoter

   T7 promotor

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET15b His6-ecDHFR (WT)

   Plasmid

   #73188

   Purpose

   bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

   Depositor

   Thomas Wandless

   Insert

   folA dihydrofolate reductase (folA E. coli)
   

   Use

   Tags

   His 6X

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET15b-His-3C-irisin

   Plasmid

   #122612

   Purpose

   Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

   Depositor

   Harold Erickson

   Article

   Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

   Insert

   irisin (ectodomain of FNDC5) (Fndc5 Mouse, Bovine, Rat, Human)
   

   Use

   Tags

   Nt: histag-3C protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 polymerase

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. wt 6XHis-SNX9 pET15b

   Plasmid

   #34690

   Depositor

   Sandra Schmid

   Article

   Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

   Insert

   Sorting nexin 9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pET15b-17X-PTDH

   Plasmid

   #166786

   Purpose

   Phosphite dehydrogenase for NAD(P)H regeneration

   Depositor

   Wilfred van der Donk

   Article

   Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

   Insert

   Phosphite dehydroenase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A196R, T201S, A328T, E352N, and C356D- please see…

   Promoter

   T7

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b LFn-Fla

    Plasmid

    #84871

    Purpose

    Bacterial expression of LFn-Fla fusion

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET15b T7pCONS sfGFP

    Plasmid

    #154467

    Purpose

    pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b LFn-Fla 3A

    Plasmid

    #84872

    Purpose

    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    3 C-terminal leucines have been replaced by alani…

    Promoter

    T7

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pet15b-Internalization Control

    Plasmid

    #186553

    Purpose

    Negative Control mediating endosomal localization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-EGFP
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-mLin28A-cHIS

    Plasmid

    #137998

    Purpose

    Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

    Depositor

    Judy Lieberman

    Article

    O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

    Insert

    mouse Lin28A, CSD and ZnFs (residues 36–186)
    

    Use

    Tags

    C-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-15b-LanCL1

    Plasmid

    #154189

    Purpose

    To express LanCL1 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 883 pET15b PTEN

    Plasmid

    #10936

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET15b+PCNA1 P186C

    Plasmid

    #66173

    Purpose

    Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    P186C

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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