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Showing: 141 - 160 of 6417 results

  3. c-Flag pcDNA3

    Plasmid
    #20011
    Purpose
    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
    Depositor
    Stephen Smale
    Article
    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20011-plasmid-map-sequence-id-372933

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  5. pHL 1075

    Plasmid
    #53004
    Purpose
    pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    fhlA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains −309 to +96 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pONSY

    Plasmid
    #111873
    Purpose
    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Type
    Empty backbone
    Use
    Capsaspora owczarzaki
    Tags
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111873-plasmid-map-sequence-id-217304

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  11. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

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  12. NK810

    Plasmid
    #196407
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196407-plasmid-map-sequence-id-385018

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  14. pHL 1073

    Plasmid
    #53003
    Purpose
    pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    ompC (target mRNA) and sodB (target mRNA)
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL 1303

    Plasmid
    #53008
    Purpose
    pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pEN-R2-mCsfGFP-L3

    Plasmid
    #178602
    Purpose
    It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.
    Depositor
    Frederica Theodoulou
    Article
    Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.
    Insert
    Tandem fluorescent protein timers (tFTs)
    Use
    Entry vector for gateway cloning
    Tags
    mCherry superfolder GFP
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    178602-plasmid-map-sequence-id-350720

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  18. pONSY-mCherry

    Plasmid
    #111874
    Purpose
    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111874-plasmid-map-sequence-id-213482

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  19. pONSY-Lifeact:mCherry

    Plasmid
    #111876
    Purpose
    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Lifeact fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111876-plasmid-map-sequence-id-213488

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  20. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

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Showing: 141 - 160 of 6417 results