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Showing: 141 - 160 of 1657 results

  1. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid
    #197265
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197265-plasmid-map-sequence-id-389723

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  2. AAVS1-eTLR

    Plasmid
    #214737
    Purpose
    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    AAVS1-eTLR (PPP1R12C Human, Rabbit, Bovine, Synthetic, Chicken, CMV, Anguilla japonica, influenza)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214737-plasmid-map-sequence-id-425005

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  3. pD649-HAsp-TIGIT-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156497
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TIGIT (TIGIT Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    156497-plasmid-map-sequence-id-368822

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  4. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Article
    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  5. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

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  6. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

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  8. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

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  12. pEXSISERS_SurfaceHaloTag_FRT-PuroR

    Plasmid
    #177869
    Purpose
    Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    Surface-HaloTag-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177869-plasmid-map-sequence-id-358833

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  13. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid
    #177868
    Purpose
    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    mGreenLantern-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    OLLAS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177868-plasmid-map-sequence-id-358838

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  14. pD649-HAsp-FAS-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156541
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FAS (FAS Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156541-plasmid-map-sequence-id-368509

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  15. p2621

    Plasmid
    #198144
    Purpose
    Epitope tagging of effector protein PipB2
    Depositor
    Michael Hensel
    Insert
    PpipB2 pipB2::M45 (STM2780 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    M45
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198144-plasmid-map-sequence-id-388085

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  16. p4295

    Plasmid
    #198153
    Purpose
    bacterial expression of pipB2::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PpipB2 pipB2::L16::HaloTag::HA (STM2780 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198153-plasmid-map-sequence-id-388088

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  17. p6173

    Plasmid
    #198157
    Purpose
    bacterial expression of sopD2::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PsopD2 sopD2 deltaCC::L16::HaloTag::HA (STM0972 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198157-plasmid-map-sequence-id-387011

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  18. p6172

    Plasmid
    #198156
    Purpose
    bacterial expression of pipB2::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PpipB2 pipB2 deltaCC::L16::HaloTag::HA (STM2780 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198156-plasmid-map-sequence-id-388057

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  20. pAP1-3

    Plasmid
    #71258
    Purpose
    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Insert
    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71258-plasmid-map-sequence-id-131888

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Showing: 141 - 160 of 1657 results