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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,917 results for: FRT

Showing: 1581 - 1600 of 1917 results

  2. pKK-TEV-EGFP

    Plasmid
    #105793
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105793-plasmid-map-sequence-id-203384

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  3. pKK-CyOFP1-TEV

    Plasmid
    #105782
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    CyOFP1-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105782-plasmid-map-sequence-id-203371

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  4. pKK-mClover3-TEV

    Plasmid
    #105778
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mClover3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105778-plasmid-map-sequence-id-203367

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  5. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid
    #140163
    Purpose
    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker
    Depositor
    Benjamin Glick
    Article
    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.
    Insert
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
    Use
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140163-plasmid-map-sequence-id-275937

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  6. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105788-plasmid-map-sequence-id-203379

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  8. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105771-plasmid-map-sequence-id-203360

    Enlarge

  9. pKK-TEV-mClover3

    Plasmid
    #105795
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mClover3
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105795-plasmid-map-sequence-id-203386

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  11. pKK-TEV-mVenus

    Plasmid
    #105791
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105791-plasmid-map-sequence-id-203382

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  14. pKK-TEV-BirA-FLAG

    Plasmid
    #105786
    Purpose
    Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-BirA-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105786-plasmid-map-sequence-id-203377

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  15. pKK-TEV-MBP

    Plasmid
    #105787
    Purpose
    Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-MBP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105787-plasmid-map-sequence-id-211379

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  18. pKK-mEGFP-TEV

    Plasmid
    #105777
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mEGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105777-plasmid-map-sequence-id-203366

    Enlarge

  20. pKK-EGFP-TEV

    Plasmid
    #105776
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105776-plasmid-map-sequence-id-203365

    Enlarge

Showing: 1581 - 1600 of 1917 results