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Showing: 1581 - 1600 of 1801 results

  1. MmXYRS-4xM15

    Plasmid
    #155343
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    Humanized MmXYRS/tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155343-plasmid-map-sequence-id-310521

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  3. optoAKT

    Plasmid
    #207164
    Purpose
    Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway
    Depositor
    Kai Zhang
    Article
    Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554
    Insert
    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207164-plasmid-map-sequence-id-413072

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  12. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

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  14. PylRS-AS

    Plasmid
    #137908
    Purpose
    For incorporation of fluorophenylalanine derivatives
    Depositor
    Wenshe Liu
    Article
    Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Containing N346A, C348S mutations
    Promoter
    pBAD
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    137908-plasmid-map-sequence-id-272329

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  20. pCS2-EcYRS-EGFP*

    Plasmid
    #191118
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Use
    Tags
    His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191118-plasmid-map-sequence-id-373723

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Showing: 1581 - 1600 of 1801 results