Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,472 results for: golden gate

Clear keywords
Showing: 1581 - 1600 of 6472 results

  3. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

    Enlarge

  10. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

    Enlarge

  11. pAV10.K3.loxP

    Plasmid
    #63202
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63202-plasmid-map-sequence-id-104899

    Enlarge

  12. pAV10.K6.loxP

    Plasmid
    #63203
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63203-plasmid-map-sequence-id-104900

    Enlarge

  13. pAV10.K5.loxP

    Plasmid
    #63204
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63204-plasmid-map-sequence-id-104903

    Enlarge

  14. pAV10.HO3.loxP

    Plasmid
    #63205
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63205-plasmid-map-sequence-id-104905

    Enlarge

  15. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

    Enlarge

  16. pAV10.HO6

    Plasmid
    #63211
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63211-plasmid-map-sequence-id-115802

    Enlarge

  18. pTwist-Kan-HC-L0-TaMOFB1(int)

    Plasmid
    #242005
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242005-plasmid-map-sequence-id-487883

    Enlarge

  19. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

    Enlarge

  20. C108m

    Plasmid
    #121010
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121010-plasmid-map-sequence-id-236173

    Enlarge

Showing: 1581 - 1600 of 6472 results