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mEmerald-MCM2-N-22

Plasmid

#54164

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
AB.pCCL.sin.cPPT.GFP.miR-218-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85881

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-218-2-3p Sensor (MIR218-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Occludin_Flag

Plasmid

#86042

Purpose

Full length human occludin

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

Occludin (OCLN Human)

Tags

FLAG

Expression

Mammalian

Mutation

I52T and Q365P

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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LentiV2-mU6-evopreQ1

Plasmid

#210190

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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G555

Plasmid

#216483

Purpose

Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

Depositor

Christophe Danelon

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

plsB, plsC, cdsA, pssA

Use

Cell-free expression

Promoter

T7 for each gene

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAJM.474

Plasmid

#108526

Purpose

LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LuxR + PLuxB-YFP

Expression

Bacterial

Mutation

VanR: T49I A111V P179S

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTPH412

Plasmid

#185724

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-gp41-8N

Use

CRISPR

Expression

Bacterial

Mutation

ecTadA(8e) R26G

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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