Addgene

We narrowed to 1,749 results for: mcherry expressing plasmid

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Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned3

Pooled Library

#101934

Purpose

Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned3. Coexpresses mCherry.

Depositor

Michael Bassik

Article

Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

Expression

Mammalian

Use

CRISPR

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned2

Pooled Library

#101933

Purpose

Bassik lab Human CRISPR-Cas9 Deletion Library - Unassigned2. Coexpresses mCherry.

Depositor

Michael Bassik

Article

Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

Expression

Mammalian

Use

CRISPR

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-FLEX-P301L Tau

Plasmid

#176267

Purpose

This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

Depositor

Tsuneya Ikezu

Article

You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

Insert

Flex-P301L tau (MAPT )

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTMB352_ZFcharm_Kv1_split-inteins

Plasmid

#220848

Purpose

Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv2

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ROSAf

Plasmid

#54323

Purpose

TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

Depositor

Silvestro Conticello

Insert

TALEN for the murine ROSA26 locus

Use

Mouse Targeting and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ROSAr

Plasmid

#54324

Purpose

TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

Depositor

Silvestro Conticello

Insert

TALEN for the murine ROSA26 locus

Use

Mouse Targeting and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMN-332

Plasmid

#195466

Purpose

Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Tags

Expression

Mutation

see attached file

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

Plasmid

#184633

Purpose

Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Aldh1a1 (Aldh1a1 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-2xStop

Plasmid

#164412

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

Plasmid

#170488

Purpose

SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Use

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Mutation

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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sgLenti-orthogonal

Plasmid

#105997

Purpose

Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS-Cas9

Plasmid

#120353

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OKMS cassette

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS-KRAB-dCas9

Plasmid

#120356

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OKMS cassette

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKG139

Plasmid

#69755

Purpose

Retroviral expression of CENP-C

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-C (CENPC Human)

Use

Retroviral

Tags

mCherry-TEV-S

Expression

Mammalian

Mutation

L341F, N570D relative to NP_001803.2 (numbering b…

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Benjamin Schuster, Daniel Hammer, Matthew Good

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR_RAF1_94

Plasmid

#111826

Purpose

Knockout Raf1

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting RAF1 94-114 (RAF1 Human)

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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