We narrowed to 31,351 results for: Eng

1. pET51b(+)-LUCIDRS-MTX

   Plasmid

   #113925

   Purpose

   Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexate

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   LUCID_RS-MTX
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7T3D-PacI-Tmeff2

   Plasmid

   #99728

   Purpose

   to make in situ RNA probes for Tmeff2

   Depositor

   Mengsheng Qiu

   Article

   Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

   Insert

   Tmeff2 (Tmeff2 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. POT5-TDH3p-ycrtYB-ADH1t

   Plasmid

   #65358

   Purpose

   TU for combinatorial construction of ?-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TDH3p-ycrtYB-ADH1t
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. POT2-TDH3p-ycrtE-ADH1t

   Plasmid

   #65352

   Purpose

   TU for combinatorial construction of ?-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TDH3p-ycrtE-ADH1t
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNLF1-C_PLCG1:1-1291

   Plasmid

   #211089

   Purpose

   Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   PLCG1:M1-L1291 (PLCG1 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   isoform 2. I813T natural variant (VAR_011908)

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiT3.1-N_FBXW7:1-707

   Plasmid

   #211099

   Purpose

   Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW7:M1-K707 (FBXW7 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBiT3.1-C_FBXW7:1-707

   Plasmid

   #211104

   Purpose

   Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW7:M1-K707 (FBXW7 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNLF1-N_CCT5:1-541

   Plasmid

   #210978

   Purpose

   Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CCT5:M1-E541 (CCT5 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNLF1-N_FBXW7:1-707

   Plasmid

   #210946

   Purpose

   Mammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW7:M1-K707 (FBXW7 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFBOH-LIC_DDB1:1-1140_delta:396-689

    Plasmid

    #210897

    Purpose

    Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    loop deletion amino acids I396 to D689

    Promoter

    Polyhedrin

    Available since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pNLF1-C_FBXW7:1-707

    Plasmid

    #210951

    Purpose

    Mammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFBOH-avi-TEV-LIC_DCAF12:1-453

    Plasmid

    #210940

    Purpose

    Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DCAF12:M1-S453 (DCAF12 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R131Q natural variant (VAR_035322)

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFBOH-MHL_EIF3I:1-325

    Plasmid

    #210910

    Purpose

    Insect expression of full-length human EIF3I. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    EIF3I:M1-A325 (EIF3I Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBiT3.1-N_DDB1:1-1140

    Plasmid

    #211147

    Purpose

    Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DDB1:M1-H1140 (DDB1 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    loop deletion I396-D689

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiT3.1-C_DDB1:1-1140

    Plasmid

    #211188

    Purpose

    Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DDB1:M1-H1140 (DDB1 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    loop deletion I396-D689

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNLF1-C_CCT5:1-541

    Plasmid

    #210979

    Purpose

    Mammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

18. EF.hICN1.CMV.GFP

    Plasmid

    #17623

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-F-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140274

    Purpose

    F-RAM, the Fos-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-N-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140275

    Purpose

    N-RAM, the Npas4-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYL-kasOp-FnCas12a2

    Plasmid

    #177874

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYL-kasOp-FnCas12a3

    Plasmid

    #177875

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized the FnCas12a mutant EP16
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.GFAP.(Cyto)iGlucoSnFR

    Plasmid

    #164513

    Purpose

    Green glucose sensor, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Insert

    (Cyto)iGlucoSnFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid

    #164514

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-INPP5K

    Plasmid

    #171950

    Purpose

    Expresses INPP5K in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5K (INPP5K Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. OA984

    Plasmid

    #120363

    Purpose

    Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

    Depositor

    Omar Akbari

    Article

    Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

    Insert

    1C19 single chain antibody
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    AAEL010782 carboxypeptidase A (CPA) gene Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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