We narrowed to 11,528 results for: Vars;

1. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

   Plasmid

   #120560

   Purpose

   Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_Nsp7

   Plasmid

   #156476

   Purpose

   Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM33_Nsp8

   Plasmid

   #156477

   Purpose

   Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. USPL1cat-C236S-pEYFP_C1

   Plasmid

   #85767

   Purpose

   mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   catalytic domain (aa 212-514), catalytic inactiv…

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX2T-TUBG1

   Plasmid

   #171967

   Purpose

   pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

   Depositor

   Maria Alvarado-Kristensson

   Article

   Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

   Insert

   TUBG1 (TUBG1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   R275H (Please see depositor comments)

   Promoter

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_Nsp3b_ADRP

   Plasmid

   #156468

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3b_ADRP (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN-puro-P2A-FRB-Fis1

   Plasmid

   #135297

   Purpose

   stably express FBR-Fis1 in mammalian cells with puromycin selection

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   FRB-Fis1 (FIS1 Human)
   

   Use

   Retroviral

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_Nsp3a_Ub1

   Plasmid

   #156467

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

   Plasmid

   #63887

   Purpose

   An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized low-affinity GCaMP3(10.19)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human SYN1

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LZRS ERTm RasN17

    Plasmid

    #21198

    Depositor

    Paul Khavari

    Article

    Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

    Insert

    HRas (Esr1, HRAS Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    S17N ERTm bx3

    Promoter

    Available Since

    June 16, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pETM33_Orf10

    Plasmid

    #156486

    Purpose

    Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. R-Q111R-N122D

    Plasmid

    #167178

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R-Q111R-N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed R at 111 to Q and changed D at 122 to N

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINF Ta-sro1 WWE-PARP

    Plasmid

    #192550

    Purpose

    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    

    Use

    Tags

    His6, 3C protease cleavage site

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6-3F2-mCherry

    Plasmid

    #73418

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3F2 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-3A2-mCherry

    Plasmid

    #73425

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3A2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3A2 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM33_Nsp3c_SUD-N

    Plasmid

    #156469

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM33_Nsp3c_SUD-M

    Plasmid

    #156470

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-5F5-mCherry

    Plasmid

    #73422

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 5F5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P5F5 (orthogonal T7-lac variant)

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. gamma-tubulin (1-335)

    Plasmid

    #226519

    Purpose

    Expressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domain

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    TUBG1 N-terminal amino acids 1-335 (TUBG1 Human)
    

    Use

    Pcdna3.1

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acids 334-451 and introduced the fo…

    Promoter

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBT-RP2.2

    Plasmid

    #189842

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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