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Plasmid#228533PurposeA fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:Rbx1:gs10:FRB:HA
Plasmid#228534PurposeHuman Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-NbON10-NanoLuc
Plasmid#198691PurposeExpresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell cultureDepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, IgK leader sequence, NanoLuc, and twin…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-LC122-TAG
Plasmid#226839PurposeContains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationamino acid 122 of light chain mutated to TAGPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-HC121-TAG
Plasmid#226836PurposeContains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationamino acid 121 of heavy chain mutated to TAGPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-HC197-TAG
Plasmid#226838PurposeContains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationamino acid 197 of heavy chain mutated to TAGPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-WT
Plasmid#215897PurposeExpression of TNRC6A-silencing domain (SD)DepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-CAMK2D
Plasmid#221686PurposeMammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioIDDepositorInsertCAMK2D (CAMK2D Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-RalA(G23C)
Plasmid#224275Purposecell transfection and expreswsion of RalA (G23C)DepositorInsertRalA (RALA Human)
UseTagsExpressionMammalianMutationG23CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8
Plasmid#221683PurposeMammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-H2-18 Heavy chain-SpyTag003
Plasmid#216307PurposeExpresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPIDAF
Plasmid#213722PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPICEAM7
Plasmid#213723PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA
Plasmid#214614Purposeadapter for RTK split TEV recruitment assaysDepositorInsert3xSH2 domains of GRB2-CTEV (GRB2 Human)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIBY55
Plasmid#213711PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only