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Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

Plasmid

#168772

Purpose

Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls (plaat1 Zebrafish)

Tags

EGFP

Promoter

alphaA-crystallin promoter

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-mApg5

Plasmid

#22958

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-mouse Plaat3 (N157stop)

Plasmid

#168771

Purpose

Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-mApg5

Plasmid

#22956

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-ER-EmEGFP

Plasmid

#169000

Purpose

Express ER-EmEGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)

Use

Unspecified

Tags

EGFP

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI3-3×FLAG

Plasmid

#215511

Purpose

Expresses FLAG tagged human WIPI3.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pRL838

Plasmid

#70680

Purpose

This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

Depositor

Peter Wolk

Article

Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

Type

Empty backbone

Use

Cre/Lox

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV mKeima-LC3

Plasmid

#189006

Purpose

Fluorescent reporter for autophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

mKeima, LC3 (Map1lc3b Rat)

Use

Retroviral

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-cfGFP

Plasmid

#200773

Purpose

Expresses cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Has Service

AAV8

Insert

cysteine-free GFP

Use

AAV

Promoter

Synapsin

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

Plasmid

#233628

Purpose

Expression of bicistronic reporter with TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)

Use

Luciferase

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-hAtg13

Plasmid

#38191

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-mApg5(K130R)

Plasmid

#22959

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCI-neo-mApg5(K130R)

Plasmid

#22957

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
PB-U6-CNCB_sgNANOG

Plasmid

#242176

Purpose

A piggybac-based vector containing mouse U6 promoter-driven NANOG sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

NANOG (NANOG Human)

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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SB-CAG-mKate2-P2A-NANOG-IP

Plasmid

#236768

Purpose

A sleeping beauty-based vector containing CAG promoter-driven mKate2-P2A-NANOG-IRES-Pac.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

NANOG (NANOG Human)

Use

Sleeping beauty

Expression

Mammalian

Promoter

CAG promoter

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-3xFLAG-His6-LARP1

Plasmid

#233626

Purpose

Expression of Flag- and His-tagged LARP1

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAB120

Plasmid

#167150

Purpose

MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

pNOS-nptII-ocsT

Expression

Plant

Promoter

pNOS

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1α-H2B-PA-mCherry-PGKneo

Plasmid

#247340

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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