Purpose
A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFĪHTH::Ī»N22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
Inserts
dxcas9 (3.7)
tetR
pspFĪHTH::Ī»N22plus
sfgfp
Tags
ASV tag and pspFĪHTH::Ī»N22plus
Mutation
Mutations D10A and H840A on WT cas9 for dCas9; Syā¦
Promoter
Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
Available Since
Sept. 23, 2019
Availability
Academic Institutions and Nonprofits only