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Addgene

We narrowed to 4,271 results for: 424

Showing: 1641 - 1660 of 4271 results
  1. Prp2-pRSETA

    Plasmid
    #61042
    Purpose
    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.
    Depositor
    Insert
    Prp2
    Use
    Tags
    Histidine Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. AKAP13

    Plasmid
    #73230
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    AKAP13 (AKAP13 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00621:RhoGEF 2002-2193, pfam00169:PH 2236-2336
    Promoter
    T7
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pEPDG1CB0010

    Plasmid
    #227573
    Purpose
    Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS
    Depositor
    Insert
    35Sshort_TMV_CYP716A392 (H424R)_35S
    Use
    Tags
    Expression
    Plant
    Mutation
    H424R, BsaI sites removed by silent mutation
    Promoter
    35Sshort_TMV (pICH51277)
    Available since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pEPDG1CB0015

    Plasmid
    #227577
    Purpose
    Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS
    Depositor
    Insert
    35Sshort_TMV_CYP716A393 (H424R)_35S
    Use
    Tags
    Expression
    Plant
    Mutation
    H424R, BsaI sites removed by silent mutation
    Promoter
    35Sshort_TMV (pICH51277)
    Available since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. LgBiT-CALM1 BiBiT-Ready Vector

    Plasmid
    #237119
    Purpose
    Express LgBiT-CALM1 Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    CALM1 (CALM1 Human)
    Use
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. LgBiT-NRAS (Q61R) BiBiT-Ready Vector

    Plasmid
    #237060
    Purpose
    Express LgBiT-NRAS (Q61R) Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Use
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. SmBiT-NRAS (Q61R) BiBiT-Ready Vector

    Plasmid
    #237061
    Purpose
    Express SmBiT-NRAS (Q61R) Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. NanoBRET Assay Vector, NanoLuc-NRAS WT

    Plasmid
    #236875
    Purpose
    Express NanoLuc(R)-NRAS WT in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pET28a-RANGAP1-CTD

    Plasmid
    #228947
    Purpose
    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized
    Depositor
    Insert
    RANGAP1-CTD (RANGAP1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    contains aa 418-587 only
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 1641 - 1660 of 4271 results