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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 1641 - 1660 of 12310 results

  1. pGCaMP6m-N3-TPC2

    Plasmid
    #80147
    Purpose
    GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time
    Depositor
    Santiago Di Pietro
    Publication
    Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPCN2 (TPCN2 Human)
    Tags
    GCaMP6m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80147-plasmid-map-sequence-id-150677

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  7. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87829-plasmid-map-sequence-id-175007

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  9. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. pcDNA3-ExRai-AktAR2

    Plasmid
    #184047
    Purpose
    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.
    Depositor
    Jin Zhang
    Publication
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AktAR2
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184047-plasmid-map-sequence-id-363388

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  15. pDEST14-Cys-SpyCatcher002

    Plasmid
    #102829
    Purpose
    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents
    Depositor
    Mark Howarth
    Publication
    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi
    Insert
    Cys-SpyCatcher002
    Tags
    GSSGS, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Cysteine residue in N-terminal region
    Promoter
    T7
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102829-plasmid-map-sequence-id-200986

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  17. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Boris Fehse
    Publication
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Mutation
    changed Asp at position 249 + 250 to Val
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60488-plasmid-map-sequence-id-96845

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  19. mCherry-Nucleus-7

    Plasmid
    #55110
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mCherry-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 1641 - 1660 of 12310 results