We narrowed to 2,714 results for: buc

1. NAX_CAGGS_CAS9_m4_EYFP

   Plasmid

   #167866

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAct:MS2-p65-HSF

   Plasmid

   #78902

   Purpose

   Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   MS2-p65-HSF
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFD3-MS2tail-SAM

   Plasmid

   #78905

   Purpose

   Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMaCTag-P04

   Plasmid

   #120015

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mTFP1
   

   Use

   Pcr template

   Tags

   mTFP1

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HOXA9 (murine) HIS-tag pET

   Plasmid

   #8492

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pMaCTag-Z18

   Plasmid

   #120061

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mKeima8.5
   

   Use

   Pcr template

   Tags

   mKeima8.5

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2CT-His-MBP-Lbu_C2c2_K1082A

   Plasmid

   #91863

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K1082A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1082A

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW216J106-Amp32C

   Plasmid

   #78651

   Purpose

   pSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tT

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   J106-30hrpR-32hrpS-t-hrpL-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

9. gRNA_tdtomato_reporter_1_ST1_2x_binding

   Plasmid

   #79368

   Purpose

   Assays activity of transcriptional activators fused to ST1 Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFD3-MS2tail-Scaffold

    Plasmid

    #78906

    Purpose

    Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMaCTag-Z04

    Plasmid

    #120047

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mTFP1
    

    Use

    Pcr template

    Tags

    mTFP1

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMaCTag-P30

    Plasmid

    #120041

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    VSV-G
    

    Use

    Pcr template

    Tags

    VSV-G

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBW214J115-Amp32C

    Plasmid

    #78649

    Purpose

    pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J115-30hrpR-32hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pMaCTag-25

    Plasmid

    #120004

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-19

    Plasmid

    #124787

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBW211J109-Amp32C

    Plasmid

    #78646

    Purpose

    pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J109-30hrpR-32hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMaCTag-P03

    Plasmid

    #120014

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxCerulean3
    

    Use

    Pcr template

    Tags

    moxCerulean3

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW213J116-Amp32C

    Plasmid

    #78648

    Purpose

    pSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J116-30hrpR-32hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3_3_TOPO_TA-ms2-VP64

    Plasmid

    #79371

    Purpose

    Recruits VP64 to a compatible gRNA for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAct:MCP-VP64

    Plasmid

    #78904

    Purpose

    Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2:VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    March 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge