We narrowed to 3,832 results for: human cdnas

1. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

   Plasmid

   #158952

   Purpose

   Express human RAD51(F129A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed phenylalanine 129 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNATM3.1/V5-His TOPO-PKCi-A120E

   Plasmid

   #55687

   Purpose

   expresses constitutively active human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E

   Promoter

   CMV

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

   Plasmid

   #69905

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (HIPK3 Fly, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

   Plasmid

   #83461

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, R4113A

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-IgL-C99-T4L-rTA

   Plasmid

   #177840

   Purpose

   Tango assay

   Depositor

   Karsten Melcher

   Article

   Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

   Insert

   C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

   Plasmid

   #228532

   Purpose

   A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

   Plasmid

   #83462

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, W4107A

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

   Plasmid

   #83460

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, S4144D

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO h-PKD1 FLS L4137P-GST

    Plasmid

    #83455

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, L4137P

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO h-PKD1 FLS R4150C-GST

    Plasmid

    #83454

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, R4150C

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2-Cx43-M100/125L-siResist

    Plasmid

    #49855

    Purpose

    Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100 and Methionine 125 mutated to Leuci…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-AU1-mTOR-S2215Y

    Plasmid

    #26037

    Depositor

    Fuyuhiko Tamanoi

    Article

    Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

    Insert

    mammalian target of rapamycin-S2215Y (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    S2215Y

    Promoter

    Available since

    Sept. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-AU1-mTOR-R2505P

    Plasmid

    #26038

    Depositor

    Fuyuhiko Tamanoi

    Article

    Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

    Insert

    mammalian target of rapamycin-R2505P (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    R2505P

    Promoter

    Available since

    Sept. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA 3.1(+) eGFP p6 K27R

    Plasmid

    #78583

    Purpose

    Expresses GFP p6 with K27R mutation in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Gurer et al J Virol. 2005 Jan;79(2):910-7.

    Insert

    eGFP p6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HIV-1 HXB2 p6 with conservative mutations (G78A, …

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

    Plasmid

    #50896

    Purpose

    Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L717C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

    Plasmid

    #50899

    Purpose

    Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A720C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

    Plasmid

    #50897

    Purpose

    Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L718C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

    Plasmid

    #50901

    Purpose

    Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L722C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)

    Plasmid

    #50895

    Purpose

    Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    S716C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge