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Showing: 2491 - 2520 of 7154 results

  1. pMCh-1480

    Plasmid
    #82426
    Purpose
    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82426-plasmid-map-sequence-id-156240

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  2. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  10. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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Showing: 2491 - 2520 of 7154 results