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NFKB3-CK-IRES-GFP hygro

Plasmid

#246914

Purpose

NFKB-dependent expression of a bicistronic cDNA encoding a suicide gene (modified deoxycytidine kinase) and EGFP

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

Mutant Deoxycytidine Kinase (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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UBC-TLR4-HA hygro

Plasmid

#246905

Purpose

Expresses HA-tagged human TLR4 in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

TLR4 (TLR4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_1k)-PGKpuro2ABFP-W

Plasmid

#208420

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_2b)-PGKpuro2ABFP-W

Plasmid

#208421

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_1k)-PGKpuro2ABFP-W

Plasmid

#208424

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_2b)-PGKpuro2ABFP-W

Plasmid

#208425

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta69-97

Plasmid

#124924

Purpose

For expression of a.a.69-97 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_WT

Plasmid

#82894

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCOG40-HRS (257-276) Hs

Plasmid

#83236

Purpose

pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

HRS (257-276) Hs (HGS Human)

Expression

Bacterial

Mutation

258-277

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutE2F(-69)-like

Plasmid

#66743

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCG (-69,-68,-67) to ATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Jacquere (all-in-one vector)

Pooled Library

#247027

Purpose

Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

Depositor

John Doench, David Root

Article

Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
Julianna (split-vector)

Pooled Library

#247028

Purpose

Enriched for effective guides through prioritization of guides with strong on-target activity. Designed against current gene annotations, offering coverage of the contemporary mouse genome.

Depositor

John Doench, David Root

Article

Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
AAV-CAG-HA-mTFEB

Plasmid

#243758

Purpose

AAV plasmid expressing HA-tagged codon-optimized mouse TFEB under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Transcription factor EB, codon optimized (Tfeb Mouse)

Use

AAV

Tags

HA tag

Promoter

CAG

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG_FBXL7

Plasmid

#171848

Purpose

pcDNA3-FLAG_FBXL7

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_S100A13_WT_V5

Plasmid

#82975

Purpose

Gateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

S100A13 (S100A13 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTetON-DHFRdd-SpvB; CMV-mCherry

Plasmid

#89463

Purpose

Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

E. coli DHFRdd destabilization domain and mCherry…

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEN6-Ubc5 Sc

Plasmid

#83234

Purpose

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Inserts

Ubiqutin (UBC Human)
Ubc 5 (UBC5 Budding Yeast)
Uba 1

Tags

His

Expression

Bacterial

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP-GFP-shch-TOG

Plasmid

#69113

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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