We narrowed to 2,013 results for: endog

1. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. N-terminal Halo-XLF

   Plasmid

   #207546

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIS1-Actb5UTR-renilla

   Plasmid

   #38234

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Actb 5'UTR / Renilla (Actb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Actb 5' UTR fused to renilla ORF

   Promoter

   Endogenous Actb

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

4. GFP-ATG8(414)/GFP-AUT7(414)

   Plasmid

   #49424

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

   Depositor

   Daniel Klionsky

   Article

   Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ATG8

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pHiUGE smFP-V5 donor ORF0

   Plasmid

   #200380

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHiUGE smFP-V5 donor ORF1

   Plasmid

   #200381

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGATOR-DTA

   Plasmid

   #64103

   Purpose

   Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

   Depositor

   Bill Skarnes

   Article

   Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

   Insert

   rTTA (Gt(ROSA)26Sor Synthetic)
   

   Use

   Mouse Targeting; Tet inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ROSA26 endogenous

   Available since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKH163_pSmart-mNEON-YIPF4

    Plasmid

    #214982

    Purpose

    endogenous mneon tag for YIPF4

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    mNEON with YIPF4 homology (YIPF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGATOR

    Plasmid

    #64102

    Purpose

    Tet inducible, Gateway adapted ROSA26 targeting Vector

    Depositor

    Bill Skarnes

    Article

    Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

    Insert

    rTTA (Gt(ROSA)26Sor Synthetic)
    

    Use

    Mouse Targeting; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ROSA26 endogenous

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. GTH-ir

    Plasmid

    #81107

    Purpose

    Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    tdiRFP
    

    Use

    Cre/Lox

    Tags

    tdiRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-GSX2-tTA-BleoR

    Plasmid

    #161748

    Purpose

    Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    Advanced tTA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    endogenous GSX2 promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GTH-y

    Plasmid

    #81105

    Purpose

    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCitrine
    

    Use

    Cre/Lox

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Mutated mCitrine for monomeric isoform (A206K L22…

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pONSY

    Plasmid

    #111873

    Purpose

    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Type

    Empty backbone

    Use

    Capsaspora owczarzaki

    Tags

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. miR-144 Trap

    Plasmid

    #60934

    Purpose

    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-144 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. GTU-g

    Plasmid

    #81094

    Purpose

    Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p.UTA.2.0-GL-2

    Plasmid

    #82440

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mutation

    The complementary region for GL2 siRNA control wa…

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. cpc-PHLS (g)

    Plasmid

    #52311

    Purpose

    Replaces the cpc operon in Synechocystis with the PHLS gene

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

    Inserts

    beta-phellandrene synthase
    Chloramphenicol resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synechocystis endogenous cpc-operon promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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