We narrowed to 32,518 results for: Eng

1. pcU6_3 MS2 sgRNA

   Plasmid

   #92394

   Purpose

   Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65449

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   tetO

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetO-hKLF4

   Plasmid

   #206386

   Purpose

   Expresses human KLF4 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB-U6-beta2 RD-1-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128343

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrnb2 (Chrnb2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai95(RCL-GCaMP6f) targeting vector

   Plasmid

   #61579

   Purpose

   Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   CAG-LSL-GCaMP6-fast
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   CAG

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-TetO-hSOX2

   Plasmid

   #206388

   Purpose

   Expresses human SOX2 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-CDreV

   Plasmid

   #140133

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   CDreV
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3-vsv-AuroraB L154G/H250Y

   Plasmid

   #108490

   Purpose

   expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   Analog sensitive L154G/H250Y

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL6 sgRNA(MS2)-MS2-hA3a D131E

   Plasmid

   #112132

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131E

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131E mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D131E mutant in hA3a

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GRAB-HaloDAmut

    Plasmid

    #234409

    Purpose

    Expresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut in neurons

    Depositor

    Yulong Li

    Article

    Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

    Insert

    GPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-GST-FBXO22

    Plasmid

    #228367

    Purpose

    express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FBXO22 (FBXO22 Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB-His-SKP1

    Plasmid

    #228368

    Purpose

    express human SKP1 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    SKP1 (SKP1 Human)
    

    Tags

    His

    Expression

    Insect

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACE-MBP-FBXL17 (310–701)

    Plasmid

    #228369

    Purpose

    express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FBXL17 (FBXL17 Human)
    

    Tags

    MBP

    Expression

    Insect

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBoBi-hPEN2 20A

    Plasmid

    #183639

    Purpose

    Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 (PSENEN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    residues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL5 sgRNA(MS2)-MS2-hA3a D131A

    Plasmid

    #112131

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131A

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131A mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D131A mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR3-vsv-AuroraB L154A/H250Y

    Plasmid

    #108489

    Purpose

    expression of vsv-AuroraB Analog sensitive (L154A/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Analog sensitive L154A/H250Y

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pvalb-2A-Cre targeting vector

    Plasmid

    #61570

    Purpose

    Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb exon 4 - 2A - Cre
    

    Use

    Cre/Lox and Mouse Targeting

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-iCreV

    Plasmid

    #140135

    Purpose

    Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iCreV
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-CBA-dt/PGK-CFTR-⌀

    Plasmid

    #163923

    Purpose

    Expresses human CFTR.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    CFTR (CFTR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-iDreV

    Plasmid

    #140136

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iDreV
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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