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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 8511 - 8520 of 9134 results

  1. prCO

    Plasmid
    #153960
    Purpose
    Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
    Use
    Retroviral; A reporter plasmid detecting dna reco…
    Tags
    NLS-ZF
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    LTR
    Available Since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153960-plasmid-map-sequence-id-306370

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  2. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

    Plasmid
    #154778
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    LaG17-SynNotch
    Tags
    LaG17
    Expression
    Mammalian
    Mutation
    204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154778-plasmid-map-sequence-id-310407

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  3. pBB0405-TEV-His12

    Plasmid
    #137040
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137040-plasmid-map-sequence-id-275693

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  4. pRevA-TEV-His12

    Plasmid
    #137034
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137034-plasmid-map-sequence-id-275692

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  5. pP13-TEV-His12

    Plasmid
    #137035
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137035-plasmid-map-sequence-id-275690

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  10. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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Showing: 8511 - 8520 of 9134 results