We narrowed to 3,178 results for: PPL

1. pTn7CdrA-gfp(ASV)C

   Plasmid

   #111617

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPAsp-hp

   Plasmid

   #48122

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   predicted optimal signal peptide for Bacillus megaterium protein secretion
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC-KIT1

   Plasmid

   #118983

   Purpose

   Mutational dissection of the G rich region of the c-kit promoter

   Depositor

   Bjorn Hogberg

   Article

   Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

   Insert

   c-kit proto-oncogene {promoter} (KIT Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

   Plasmid

   #110554

   Purpose

   miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Expression

   Bacterial

   Promoter

   AraC-ParaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC-KIT8

   Plasmid

   #118990

   Purpose

   Mutational dissection of the G rich region of the c-kit promoter

   Depositor

   Bjorn Hogberg

   Article

   Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

   Insert

   c-kit proto-oncogene {promoter} (KIT Human)
   

   Expression

   Mammalian

   Mutation

   mutK2-mutSP-mutK1

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. H_NES 41kDa

   Plasmid

   #201346

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   HIV_NES-EGFP-2PrA
   

   Expression

   Mammalian

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MT363

   Plasmid

   #65858

   Purpose

   The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

   Depositor

   Detlef Weigel

   Article

   Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

   Insert

   nahG
   

   Expression

   Plant

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV::TVA/tRFP

    Plasmid

    #164486

    Purpose

    Dual CMV promoter plasmid to express TVA and TurboRFP

    Depositor

    Hollis Cline

    Article

    Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

    Inserts

    TVA (CD320 Chicken)
    TurboRFP
    

    Expression

    Mammalian

    Promoter

    sCMV and sCMV IE94

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA-ura-HYB

    Plasmid

    #64330

    Purpose

    ura3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of ura3 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMpGE010

    Plasmid

    #71536

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX301-ZIM3-KRAB-PYL1

    Plasmid

    #154761

    Purpose

    Expresses ZIM3 KRAB domain fused to PYL1

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSum-LacZ-gfp

    Plasmid

    #109285

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GFP
    

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    LacZalpha

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

    Plasmid

    #110572

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD-stRBS-aacC1
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSPPac-hp

    Plasmid

    #48123

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of Pac
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBECKP-spe

    Plasmid

    #117236

    Purpose

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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