161,273 results

1. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

   Plasmid

   #229828

   Purpose

   A Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Puro_siKD

   Plasmid

   #86695

   Purpose

   Tetracycline inducible expression of shRNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 TO

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL056

   Plasmid

   #177168

   Purpose

   Expresses wildtype MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176624

   Purpose

   TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

   Viral Prep

   #100849-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (#100849). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   CAG

   Tags

   None

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pUC19_HDRT_TRAC_CD19-CAR

   Plasmid

   #183473

   Purpose

   Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

   Inserts

   TRAC (TRAC Human)
   CD19-specific CAR transgene insert
   TRAC (TRAC Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE6f

   Plasmid

   #207856

   Purpose

   Mammalian expression of PE6f prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6f
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTP1172

   Plasmid

   #104161

   Purpose

   Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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