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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,586 results for: 112

Showing: 1721 - 1740 of 11586 results

  1. pRTC2-puro

    Plasmid
    #190567
    Purpose
    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    L1 (L1.3) with reporter cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190567-plasmid-map-sequence-id-393296

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  7. mCerulean-hCOL1A1

    Plasmid
    #140112
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140112-plasmid-map-sequence-id-296778

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  10. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  12. pRT801

    Plasmid
    #68112
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68112-plasmid-map-sequence-id-248995

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  17. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Use
    Tags
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

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  18. pUASTattb-cAMPr Y205A, R626A

    Plasmid
    #84932
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R626A
    Use
    Tags
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R626A
    Promoter
    5X UAS
    Available since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84932-plasmid-map-sequence-id-180302

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Showing: 1721 - 1740 of 11586 results