We narrowed to 7,661 results for: Ski

1. pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

   Plasmid

   #113693

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 gRNA Cassete
   

   Use

   AAV and CRISPR

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-jYCaMP1

   Plasmid

   #135423

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Insert

   jYCaMP1
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

   Plasmid

   #113679

   Purpose

   A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   NLS and VP64

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MT-AvrXa10-FT

   Plasmid

   #234373

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

   Depositor

   Michael Smanski

   Article

   Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

   Inserts

   NbGP41-GFP-AvrXa10-GB1
   dCas9-24XGP41
   gRNAs targeting FT locus
   RFP
   

   Use

   Synthetic Biology

   Tags

   GB1, GFP, and GP41

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBetaActin-FKBP-mScarlet-Dync1h1motor

   Plasmid

   #191330

   Purpose

   Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
   

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-FHH-NRASQ61K-IRES-Puro

   Plasmid

   #120570

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

   Plasmid

   #233039

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d
   

   Use

   AAV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-FHH-NRAS-IRES-Puro

   Plasmid

   #120569

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-GG-OCT4-1-5-PGK-Puro

   Plasmid

   #102893

   Purpose

   PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-FLAG-NRASQ61K-IRES-Puro

    Plasmid

    #120575

    Purpose

    Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-jYCaMP1s

    Plasmid

    #135424

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

    Plasmid

    #113681

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1/mCherry-DMDEx23

    Plasmid

    #211367

    Purpose

    Expresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)

    Depositor

    Ulrich Lächelt

    Article

    Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

    Insert

    mCherry-DMDEx23
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mCherry interrupted by mdx dystrophin exon 23 bet…

    Promoter

    CMV

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

    Plasmid

    #113678

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3045

    Plasmid

    #144521

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SNW1 (SNW1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rat SERCA2a (pMT2)

    Plasmid

    #75183

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, Gary Shull

    Article

    Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

    Insert

    ATP2A2
    

    Expression

    Mammalian

    Promoter

    adenovirus major late promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. CIBN-rTetR

    Plasmid

    #183919

    Purpose

    Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    CIBN-rTetR (CIB1 Synthetic, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a Cdk2ap1CAN

    Plasmid

    #178032

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Promoter

    T7LacI

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-D4 miR30-AGO2 ts1

    Plasmid

    #115853

    Purpose

    AGO2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO2 shRNA (AGO2 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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