161,296 results

1. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MCS+

   Plasmid

   #90412

   Purpose

   A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane

   Depositor

   Katharina Gaus

   Article

   Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

   Insert

   Lck10+ Venus+mcherry+ strech of positively charged amino acids
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-Rab5DN(S34N)

   Plasmid

   #35139

   Depositor

   Sergio Grinstein

   Article

   Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

   Insert

   Rab5a (RAB5A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Dominant Negative- S34N

   Promoter

   CMV

   Available since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDB5321

   Plasmid

   #204831

   Purpose

   An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW3 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

   Plasmid

   #32703

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Cre/Lox and Retroviral

   Tags

   3x HA tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. piRFP

   Plasmid

   #31857

   Purpose

   a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

   Has Service

   Cloning Grade DNA

   Insert

   iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

   Promoter

   CMV

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYSD085

   Plasmid

   #212922

   Purpose

   Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   649 Stalk
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Use

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEG302 22aa SunTag NtDRMcd nog

   Plasmid

   #115488

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP Cortactin

    Plasmid

    #50728

    Purpose

    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cortactin (CTTN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge