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We narrowed to 7,046 results for: tet on

Showing: 1741 - 1760 of 7046 results

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TetO-FUW-FLAG-NLS-VP64-MCS_N-term

Plasmid

#136886

Purpose

Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

VP64 activator domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

Plasmid

#136888

Purpose

Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

Plasmid

#121804

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

Plasmid

#121808

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L900A(pL91)

Plasmid

#124395

Purpose

E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L900A (TET1 Human)

Use

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L900A

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898E(pL91)

Plasmid

#124394

Purpose

E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898E (TET1 Human)

Use

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898E

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898A(pL91)

Plasmid

#124393

Purpose

E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898A (TET1 Human)

Use

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898A

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L897A(pL91)

Plasmid

#124392

Purpose

E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L897A (TET1 Human)

Use

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L897A

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-I894A(pL91)

Plasmid

#124391

Purpose

E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-I894A (TET1 Human)

Use

Tags

GB1-Histidine

Expression

Bacterial

Mutation

I894A

Promoter

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

Plasmid

#91874

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)

Plasmid

#70733

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)

Plasmid

#70739

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-off Full length Tau (0N4R, P301L/V337M)

Plasmid

#188573

Purpose

Dox-repressible expression of the 0N4R Tau isoform

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

0N4R Tau (MAPT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

Plasmid

#184395

Purpose

Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

Tet-On 3G

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

Plasmid

#184396

Purpose

Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

Tet-On 3G

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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