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Showing: 1741 - 1760 of 65764 results

  3. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid
    #66707
    Purpose
    expresses a single gRNA and VP64-dCas9-VP64
    Depositor
    Charles Gersbach
    Inserts
    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hU6 and hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66707-plasmid-map-sequence-id-244291

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  5. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Article
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    102562-plasmid-map-sequence-id-203161

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  12. PDIA3-bio-His

    Plasmid
    #52070
    Purpose
    Expresses full-length Protein disulfide-isomerase A3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PDIA3 (PDIA3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52070-plasmid-map-sequence-id-270198

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  13. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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  15. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Insert
    SARS-2 Spike Protein (S Human, SARS-COV-2, Synthetic)
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Promoter
    standard CMV
    Available Since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

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  17. mCherry-EB3-7

    Plasmid
    #55037
    Purpose
    Localization: MT End Binding Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    EB3 (MAPRE3 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55037-plasmid-map-sequence-id-269079

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  18. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available Since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  20. pcDNA3.3_CoV2_D18

    Plasmid
    #170442
    Purpose
    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    Tags
    NA
    Expression
    Mammalian
    Mutation
    18aa deletion in c-terminal tail
    Promoter
    CMV
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170442-plasmid-map-sequence-id-337888

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Showing: 1741 - 1760 of 65764 results