We narrowed to 5,840 results for: pCas

1. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-opCas12a-Ultra-6xNLS

   Plasmid

   #209024

   Purpose

   Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/M537R/S542R/F870L

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-deSpCas9-VP64-6xHis

   Plasmid

   #92117

   Purpose

   Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Bgh-GFP-Farpl

   Plasmid

   #115501

   Purpose

   expression of rat Farp1

   Depositor

   Thomas Biederer

   Article

   Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

   Insert

   Farp1 (Farp1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   chicken beta-actin

   Available Since

   Dec. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-SARS2-S-FKO (NC-flag)

   Plasmid

   #159363

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Tags

   Flag and Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-SARS2-S-G614 (NC-flag)

   Plasmid

   #156419

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 35S::Cel1SP-GeNL/pCAMBIA1301

   Plasmid

   #190069

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Cel1 signal peptide-Green-enhanced Nano-lantern
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-Flag-hsDicer (E1564A)

    Plasmid

    #41587

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E1564A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    D156R, R883K, R887A

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCASP-SptP120-3G124-HilA

    Plasmid

    #153323

    Purpose

    Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3G124 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT344_pattB-pCA-GFP-pA-FRT5

    Plasmid

    #52550

    Purpose

    for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCaSpeR4 mito roGFP2-Grx1

    Plasmid

    #65000

    Purpose

    Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-PCAF (delta5-53a.a.)

    Plasmid

    #65388

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    PCAF (KAT2B Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    lost 13-159bp of the ORF

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCAGGS-Flag-hsDicer (D1320A)

    Plasmid

    #41586

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1320A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-attBr-Cre-rc-attP

    Plasmid

    #51264

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-Cre-rc-attP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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