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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,803 results for: pDest

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Showing: 1741 - 1760 of 1803 results

  5. A1-LCD allW D262V

    Plasmid
    #234616
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234616-plasmid-map-sequence-id-469043

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  6. A1-LCD allW D262N

    Plasmid
    #234615
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234615-plasmid-map-sequence-id-469028

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  7. A1-LCD-2R-2K+3D

    Plasmid
    #178680
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-2R+2K+3D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    two Arg residues removed, two Lys added, three As…
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178680-plasmid-map-sequence-id-356258

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  8. A1-LCD-9F+3Y

    Plasmid
    #178663
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+3Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, three Tyr added
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178663-plasmid-map-sequence-id-356435

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  9. A1-LCD-14N+14Q

    Plasmid
    #178692
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-14N+14Q (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    fourteen Gln residues replaced with Asn
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178692-plasmid-map-sequence-id-356415

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  11. A1-LCD+7K+12D

    Plasmid
    #178679
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7K+12D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Lys residues added, twelve Glu residues add…
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178679-plasmid-map-sequence-id-356398

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  12. A1-LCD-30G+30S

    Plasmid
    #178684
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178684-plasmid-map-sequence-id-356310

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  13. A1-LCD+23G-23S

    Plasmid
    #178685
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178685-plasmid-map-sequence-id-356307

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  14. A1-LCD-4R-2K+5D

    Plasmid
    #178681
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-4R+2K+5D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    four Arg residues removed, two Lys added, five As…
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178681-plasmid-map-sequence-id-356295

    Enlarge

  15. A1-LCD-9F+6Y

    Plasmid
    #178661
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+6Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, six Tyr added
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178661-plasmid-map-sequence-id-356293

    Enlarge

  16. A1-LCD-8F+4Y

    Plasmid
    #178662
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-8F+4Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    eight Phe residues removed, four Tyr added
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178662-plasmid-map-sequence-id-356201

    Enlarge

  17. A1-LCD+7R+10D

    Plasmid
    #178677
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7R+10D (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, ten Glu residues added
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178677-plasmid-map-sequence-id-356191

    Enlarge

  18. A1-LCD-12F+12Y

    Plasmid
    #178659
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD -12F+12Y (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twelve Phe residues replaced with Tyr
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178659-plasmid-map-sequence-id-356187

    Enlarge

  19. A1-LCD-23S+23T

    Plasmid
    #178693
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-23S+23T (HNRNPA1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Thr reisudes replaced with Ser
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    178693-plasmid-map-sequence-id-356186

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Showing: 1741 - 1760 of 1803 results