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pmTurquoise2-LAMP1

Plasmid

#110948

Purpose

Expresses LAMP1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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Calnexin-HT8-mGold

Plasmid

#183989

Purpose

Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

Calnexin (CANX Human)

Tags

HaloTag8 (C61S, C262S), mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

Plasmid

#217719

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR3 (CELSR3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2522

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pATZ-Myc

Plasmid

#177718

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

Myc

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173864

Purpose

Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

Plasmid

#217713

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA3 (ADGRA3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-743

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

Plasmid

#217712

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA2 (ADGRA2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-752

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

Plasmid

#217737

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR6 (ADGRG6 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-846

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-B/c

Plasmid

#227962

Purpose

Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

Plasmid

#217740

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL2 (ADGRL2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-830

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

Plasmid

#217717

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR1 (CELSR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2453

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

Plasmid

#217715

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB2 (BAI2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-917

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

Plasmid

#217743

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5896

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG

Plasmid

#217742

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL4 (ADGRL4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-412

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG

Plasmid

#217716

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-862

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG1 CTFdeltaTA-FLAG

Plasmid

#217732

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG1 (ADGRG1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-388

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

Plasmid

#217718

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR2 (CELSR2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2362

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD1 CTFdeltaTA-FLAG

Plasmid

#217720

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRD1 (ADGRD1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-550

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

Plasmid

#217735

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2727

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG

Plasmid

#217733

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG2 (ADGRG2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-612

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-HA-GFP11-KDEL

Plasmid

#177722

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

GFP11, HA, KDEL, and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-AgDD

Plasmid

#80625

Purpose

create nuclear protein aggregates in mammalian cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NLS-NES-L106P-sfGFP (FKBP1A Human)

Tags

MPPKKKRKVLALKLAGLDI

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173869

Purpose

Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pATZ-HA-GFP11

Plasmid

#177720

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge