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pET:CCCT:GFP

Plasmid

#60738

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMEP5-Flag Par6 S345A

Plasmid

#24648

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

Par6 (Pard6a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S345A

Available Since

June 2, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pLKO.1-blast-cfRab8b_3

Plasmid

#26709

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8b_3 RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-Blast-cfCdc42_3

Plasmid

#26704

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Cdc42 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Cdc42 RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-Flag-mPar6a.PIG

Plasmid

#24599

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101106

Purpose

Encodes FKBP-dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-FKBP-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1-lambdaN-HA-DmDCP1

Plasmid

#21685

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pME-fli1b

Plasmid

#49019

Purpose

full length coding sequence for zebrafish fli1b gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1b (fli1rs Zebrafish)

Use

Gateway middle entry

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCis_7+8+10

Plasmid

#60588

Purpose

a 3-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8+10

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Plasmid

#137118

Purpose

Protease Switch Clone ID: 2-B3

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Use

Synthetic Biology

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch13_102010574-gRNA-for

Plasmid

#81218

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch13_102010574-gRNA-rev

Plasmid

#81219

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEYC1A

Plasmid

#127758

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in convergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI134

Plasmid

#126071

Purpose

Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI135

Plasmid

#126073

Purpose

Vector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCVD468

Plasmid

#66155

Purpose

expression of His-tagged espA

Depositor

James Kaper

Article

Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

Insert

espA

Tags

His

Expression

Bacterial

Promoter

T5

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pET11a-TAX-L10-STEPtag

Plasmid

#159375

Purpose

Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

TAX-L10-STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag-L10-TAX

Plasmid

#159374

Purpose

Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag-L10-TAX

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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