We narrowed to 2,218 results for: dcas9

1. pOP593

   Plasmid

   #85950

   Purpose

   pOP576 with gRNA key

   Depositor

   Timothy Lu

   Article

   Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

   Insert

   dCas9 and gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOP594

   Plasmid

   #85951

   Purpose

   pOP576 with gRNA key

   Depositor

   Timothy Lu

   Article

   Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

   Insert

   dCas9 and gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Promoter

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGDestX2-Amp

   Plasmid

   #157654

   Purpose

   Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti sgRNA(MS2) mCherry

   Plasmid

   #171053

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   sgRNA(MS2) mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-Basic-8x-gRNA-eGFP

   Plasmid

   #60718

   Purpose

   eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSABEd

   Plasmid

   #133968

   Purpose

   Expresses ABEd CRISPR base editor and gRNA scaffold in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Zhong et al bioRxiv 630137

   Insert

   ABEd
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN-PTet-YFP

   Plasmid

   #62245

   Purpose

   used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN-PBAD-YFP

    Plasmid

    #62246

    Purpose

    used to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-dSa VP64 Afp

    Plasmid

    #99697

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPRoff-mScarletI

    Plasmid

    #217783

    Purpose

    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    

    Use

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-BE2

    Plasmid

    #73020

    Purpose

    Expresses BE2 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVP144

    Plasmid

    #222081

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and eforRED
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVP143

    Plasmid

    #222080

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVP141

    Plasmid

    #222079

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP and sacB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti pTERT sgRNA1(MS2) mCherry

    Plasmid

    #171054

    Purpose

    Targeting of Vp64-dCas9 activation system to hTERT promoter

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    pTERT sgRNA1(MS2) mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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