221,377 results

1. pAAV-Ef1a-fDIO mCherry (AAV9)

   Viral Prep

   #114471-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry (Flp-dependent)

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

2. TDP-43 mRuby

   Plasmid

   #216225

   Purpose

   TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK DN RhoA

   Plasmid

   #84646

   Purpose

   Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   RhoA (RHOA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Dominant Negative

   Promoter

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

4. TRBV5-1

   Plasmid

   #123414

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV5-1 (TRBV5-1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP1muA-TurboID-polyA-G418 HR

   Plasmid

   #227734

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInt-ts

   Plasmid

   #66076

   Purpose

   The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   lambda integrase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0040

   Plasmid

   #143684

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF676 (ZNF676 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-DNMT3A-PuroR (ANV)

   Plasmid

   #71684

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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