165,173 results

1. AASDHPPT

   Plasmid

   #39192

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AASDHPPT (AASDHPPT Human)
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

   Plasmid

   #105648

   Purpose

   Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

   Depositor

   Sandra Rieger

   Insert

   Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-rm_gCarvi-T2A

   Plasmid

   #190334

   Purpose

   Ratiometric reporter for cAMP

   Depositor

   Naoto Saitoh

   Article

   Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   ratiometric gCarvi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28GSTlinker-LIC

   Plasmid

   #119970

   Purpose

   Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. UKNP1.9.1

   Plasmid

   #97374

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. C3AR1-Tango

   Plasmid

   #66231

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   C3AR1 (C3AR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84241

   Purpose

   Expresses ABA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC70188

   Plasmid

   #209449

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   HvU3

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-hygro-STING HAQ

   Plasmid

   #102600

   Purpose

   Express STING HAQ for IFN-beta Luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   R71H-G230A-R293Q (HAQ)

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cterminal HA WDR59 pRK5

    Plasmid

    #46328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR59 (WDR59 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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