164,599 results

1. pJR104

   Plasmid

   #187243

   Purpose

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-BHLHE40

   Plasmid

   #110154

   Purpose

   Expression of human BHLHE40 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-spRYCas9-ABE-UMax

   Plasmid

   #222139

   Purpose

   Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   TadA-nSpRYCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-MTOR-S2215Y

   Plasmid

   #69013

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2215Y

   Promoter

   CMV

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-2E2-HA-mEGFP

   Plasmid

   #129595

   Purpose

   The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-Flpo (AAV1)

   Viral Prep

   #55637-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Tags

   None

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pRSV-Rev

   Plasmid

   #12253

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   Rev (rev )
   

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV GFP DEST (736-1)

   Plasmid

   #19732

   Purpose

   3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmal_c5x_RNAse_Inhib

    Plasmid

    #153314

    Purpose

    Bacterial expression of an RNase inhibitor

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    RNAse Inhibitor
    

    Use

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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