We narrowed to 475 results for: chat

1. pcDNA3.1-AntiHer2-HC197-TAG

   Plasmid

   #226838

   Purpose

   Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 197 of heavy chain mutated to TAG

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcBac1-EcTrp-TGA

   Plasmid

   #228780

   Purpose

   aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpRYc-ABE8e-EGFP

   Plasmid

   #208340

   Purpose

   Expresses SpRYc-ABE8e in mammalian cells

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   ecTadA(8e)-nSpRYc
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CMV

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA228-pro4IUPi

   Plasmid

   #122018

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pro4

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-CAG-F14F15S-Switch

   Plasmid

   #99733

   Purpose

   Flp-based FLEX reporter (from RFP to GFP)

   Depositor

   Ian Wickersham

   Article

   Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

   Insert

   TagRFP and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac1-BacMam-4x-PyOtR

   Plasmid

   #217364

   Purpose

   Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   4 copies of pyrrolysyl-tRNA mutant PyOtR
   

   Use

   Baculoviral

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-ITR-PytR(A2.1)-mCherry

   Plasmid

   #204869

   Purpose

   Expresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA mutant "A2.1"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4G, T65C, T66C, T67T

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-ITR-EcYtR(NGS-6)-mCherry

   Plasmid

   #217363

   Purpose

   Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA mutant, "NGS-6"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTET-IUPi

   Plasmid

   #122019

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pTET

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIDTSmart-CMV-PLRS1

    Plasmid

    #217365

    Purpose

    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    leucyl-tRNA synthetase polyspecific mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M40I, T252A, Y499I, Y527A, H529G

    Promoter

    CMV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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