We narrowed to 24,015 results for: pET

1. pET24a_SMOXdel

   Plasmid

   #186785

   Purpose

   C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

   Insert

   Spermine oxidase (SMOX Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 503-512

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCyPet-C1

   Plasmid

   #22779

   Depositor

   Klaus Hahn

   Article

   Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

   Insert

   CyPet
   

   Expression

   Mammalian

   Mutation

   Control for intermolecular Rac1 biosensor FRET. S…

   Available Since

   Dec. 18, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pET_BGL1

   Plasmid

   #101910

   Purpose

   Expresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7+LacO

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET22b_EcSSB_1

   Plasmid

   #78203

   Purpose

   Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

   Depositor

   Martin Webb

   Article

   Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

   Insert

   ssb (G26C)
   

   Expression

   Bacterial

   Mutation

   G26C (please see depositor comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ETV2_pet28a

   Plasmid

   #131665

   Purpose

   Bacterial expression of ETS gene ETV2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV2 (ETV2 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_Nsp9

   Plasmid

   #156478

   Purpose

   Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp9 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET14b_tev_PSG

   Plasmid

   #59731

   Purpose

   Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria

   Depositor

   Alan Fanning

   Article

   Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

   Insert

   TJP1 (TJP1 Human)
   

   Tags

   HIS and TEV

   Expression

   Bacterial

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. ELK3_pet28a

   Plasmid

   #131651

   Purpose

   Bacterial expression of ETS gene ELK3

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELK3 (ELK3 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYpet-C1

    Plasmid

    #22780

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Ypet
    

    Expression

    Mammalian

    Mutation

    Control for intermolecular Rac1 biosensor FRET. S…

    Available Since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pET11a_PTK2b_Kinase

    Plasmid

    #224242

    Purpose

    Expression of human PTK2b Kinase domain in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b_kinase (PTK2B Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CYPet-N1

    Plasmid

    #54638

    Purpose

    Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Type

    Empty backbone

    Tags

    CYPet

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET28CLY5

    Plasmid

    #21764

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pET26_MraYaa

    Plasmid

    #100166

    Purpose

    recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Chung et al Science. 2013 Aug 30;341(6149):1012-1016

    Insert

    mraY (phospho-MurNAc-pentapeptide translocase)
    

    Tags

    MBP-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET_BGL2

    Plasmid

    #101911

    Purpose

    Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.

    Depositor

    Steve Shih

    Article

    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

    Insert

    β-glucosidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7+LacO

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28Teb1

    Plasmid

    #28173

    Depositor

    Kathleen Collins

    Article

    Min et al Mol Cell. 2009 Nov 25. 36(4):609-19.

    Insert

    Teb1
    

    Tags

    6xhistidine

    Expression

    Bacterial

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. FEV_pet28a

    Plasmid

    #131668

    Purpose

    Bacterial expression of ETS gene FEV

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FEV (FEV Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM33_Nsp3d_Ub2_PLPro

    Plasmid

    #156472

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3d_Ub2_PLPro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_LolT_C41S

    Plasmid

    #211789

    Purpose

    Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

    Depositor

    Yang Hai

    Article

    Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

    Insert

    lolt mutant
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET11d_Rev

    Plasmid

    #119322

    Purpose

    Expression of protein Rev from HIV

    Depositor

    Francisco Blanco, Robert Tycko

    Article

    Blanco et al J Mol Biol. 2001 Nov 2;313(4):845-59. do

    Insert

    Rev (rev HIV B1)
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET22b_EcSSB_2

    Plasmid

    #78204

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-

    Insert

    ssb (W88C)
    

    Expression

    Bacterial

    Mutation

    W88C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ELF3_pet28a

    Plasmid

    #131658

    Purpose

    Bacterial expression of ETS gene ELF3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF3 (ELF3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ETV1_pet28a

    Plasmid

    #131644

    Purpose

    Bacterial expression of ETS gene ETV1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV1 (ETV1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pet15b_ack

    Plasmid

    #220229

    Purpose

    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    Acetate kinase (ackA )
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ETS2_pet28a

    Plasmid

    #131664

    Purpose

    Bacterial expression of ETS gene ETS2

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETS2 (ETS2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ELF4_pet28a

    Plasmid

    #131661

    Purpose

    Bacterial expression of ETS gene ELF4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF4 (ELF4 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22B_PcrA_2Bchimera

    Plasmid

    #102998

    Purpose

    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

    Depositor

    Martin Webb

    Insert

    PcrA_2Bchimera
    

    Expression

    Bacterial

    Mutation

    Bacilllus stearothermophilus PcrA in which the 2B…

    Promoter

    T7

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETM33_Nsp1

    Plasmid

    #156465

    Purpose

    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CYPet-C1

    Plasmid

    #54649

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Type

    Empty backbone

    Tags

    CYPet

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits