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pEGFP-C1-BBS1
Plasmid#218711PurposeExpresses N-terminally EGFP-tagged BBS1 in mammalian cellsDepositorInsertBBS1 (BBS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]
Plasmid#86455PurposeNuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa
Plasmid#86452PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFsDepositorInsertUAS-Tc'hsp_p-tGFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
NoLS-Grx1-roGFP2
Plasmid#236545PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleoli.DepositorInsertNoLS-Grx1-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)
Plasmid#160634PurposeTU for wtGFP expression driven by 2x35s promoter.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter2x35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
NET29b.Mm pEGFP-N2 (1327)
Plasmid#62046Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET29b (Tmem120b Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSquash_B
Plasmid#145926PurposeInsect Expression of DmSquashDepositorInsertDmSquash (squ Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTcTurboID-GW_GFP-NLS
Plasmid#234716PurposeUsed to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.DepositorInsertGFP-3xNLS
UseTrypanosomatid expressionTags3xHAExpressionMutationPromoterT7Available sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmYTHDFa_AB
Plasmid#148291PurposeInsect Expression of DmYTHDFaDepositorInsertDmYTHDFa (CG6422 Fly)
UseTagsExpressionInsectMutation1 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsSmaug2_Q
Plasmid#147329PurposeMammalian Expression of HsSmaug2DepositorInsertHsSmaug2 (SAMD4B Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the seqeuence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRpL10_R
Plasmid#147348PurposeInsect Expression of DmRpl10DepositorInsertDmRpl10 (RpL10 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSS-SGFP2-N1
Plasmid#112938PurposeVisualization of F-actinDepositorInsertLifeAct
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
KIF3A560GFP
Plasmid#129769PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKIF3A (Kif3a Mouse)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTre-Tight-luciferase-GFP (467)
Plasmid#65491Purposenegative control for expression assaysDepositorInsertEGFP
UseLuciferaseTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-splitTVA-EGFP
Plasmid#59332PurposeExpresses splitTVA-P2A-EGFPDepositorInsertsplitTVA950-P2A-EGFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-EGFP-B19G
Plasmid#59333PurposeExpresses EGFP-P2A-B19GDepositorInsertEGFP-P2A-B19G
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterSynapsin-1Available sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-DIO-TC66T-2A-EGFP-2A-oG
Plasmid#178429PurposeExpresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsTVA950 Glu66→Thr
Optimized G
EGFP
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationPromoterhSynAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdIDR7-H1
Plasmid#221930PurposeTransient expression of GFP-tagged CBPdeltaIDR7-H1 in mammalian cellsDepositorInsertCBPdeltaIDR7-H1 (CREBBP Human)
UseTagsGFPExpressionMammalianMutationdeletion of IDR7, CBP aa 2187 - 2216PromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdCFID
Plasmid#221909PurposeTransient expression of GFP-tagged CBPdeltaCFID in mammalian cellsDepositorInsertCBPdeltaCFID (CREBBP Human)
UseTagsGFPExpressionMammalianMutationdeletion of the CBP-FUS interaction domain (CFID)…PromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-N1_CBPdIDR6-H2
Plasmid#221929PurposeTransient expression of GFP-tagged CBPdeltaIDR6-H2 in mammalian cellsDepositorInsertCBPdeltaIDR6-H2 (CREBBP Human)
UseTagsGFPExpressionMammalianMutationdeletion of IDR6, CBP aa 1951 - 1978PromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
p007ampGFP
Plasmid#58535PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p006kanGFP
Plasmid#58534PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-FLuc-K21-Rluc_AH
Plasmid#148847PurposeMammalian Expression of K21-RlucDepositorInsertK21-Rluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUAL OCLN (GFP)
Plasmid#86982PurposeLentiviral expression construct encoding Occludin and GFP from separate promotersDepositorInsertOCLN (OCLN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
NET29A.Mm pEGFP-N2 (1326)
Plasmid#62045Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET29A (Tmem120a Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMTOR_AA
Plasmid#148202PurposeInsect Expression of DmMTORDepositorInsertDmMTOR (Mtor Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RIX-F2Oct4F3-GFP
Plasmid#114313PurposeTissue-specific expression of the GFP living color under the control of the Oct4 promoterDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLX-Ngn3-GFP
Plasmid#114309PurposeTissue-specific expression of the GFP living color under the control of the Ngn3 promoterDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLX-KRT7-GFP
Plasmid#114312PurposeTissue-specific expression of the GFP living color under the control of the Keratin7 promoterDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPA32-EGFP
Plasmid#211383Purposemammalian expression of human RPA32 C-terminally fused to EGFPDepositorInsertRPA32 (RPA2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Cited2
Plasmid#112246Purposeexpression of EGFP-Cited2 in mammalian cellsDepositorInsertCited2 (CITED2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
hKv2.1-pEGFP-C1
Plasmid#111538PurposeExpresses human KCNB1 in mammalian cells for imagingDepositorInserthuman Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RPA32
Plasmid#211381Purposemammalian expression of human RPA32 N-terminally fused to EGFPDepositorInsertRPA32 (RPA2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only