We narrowed to 1,068 results for: golden gate assembly

Showing: 401 - 450 of 1068 results

Results per page:

Sort results by:

p5E_GGWDest-

Plasmid

#49318

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_GGWDest+

Plasmid

#49321

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGDestSC-ATG

Plasmid

#49322

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E_GGWDest+

Plasmid

#49317

Purpose

Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pME_GGWDest-

Plasmid

#49320

Purpose

Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
GGDest2-Amp

Plasmid

#157650

Purpose

Golden Gate assembly of a pET28-compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GGDest3-Amp

Plasmid

#157651

Purpose

Golden Gate assembly of a pTXB1 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGA-red-maxi

Plasmid

#196337

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(I) pXYL (J) P3 (SBE108)

Plasmid

#194991

Purpose

promoter of the gene xylose reductase from R. toruloides for a position P3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pXYL (J)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(J) crtYB(K) G3 (SBE117)

Plasmid

#194996

Purpose

Rhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(J) crtYB(K)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(G) crtI (H) G2 (SBE116)

Plasmid

#194995

Purpose

Rhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(G) crtI (H)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(D) crtE (E)_G1 (SBE115)

Plasmid

#194994

Purpose

Rhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(D) crtE (E)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGA-red-mini

Plasmid

#196338

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT-HAB1-backbone

Plasmid

#241287

Purpose

Backbone for HAB1 mutants library construction from oligopools; PYR1 sensor library assembly from oligopools;

Depositor

Sean Cutler

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDOC-GG

Plasmid

#149377

Purpose

Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Inserts

Fragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA3

Plasmid

#158481

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA16

Plasmid

#158494

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, no STOP codon

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA2

Plasmid

#158480

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA11

Plasmid

#158489

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA9

Plasmid

#158487

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA4

Plasmid

#158482

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA10

Plasmid

#158488

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA1

Plasmid

#158479

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_5'_Linker

Plasmid

#49291

Purpose

Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_3'_Linker

Plasmid

#49289

Purpose

Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_2'_XcmI-LacZ

Plasmid

#49303

Purpose

Golden Gate entry vector at position 2, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_3'_XcmI-LacZ

Plasmid

#49304

Purpose

Golden Gate entry vector at position 3, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_4'_XcmI-LacZ

Plasmid

#49305

Purpose

Golden Gate entry vector at position 4, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_5'_XcmI-LacZ

Plasmid

#49306

Purpose

Golden Gate entry vector at position 5, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_6'_XcmI-LacZ

Plasmid

#49307

Purpose

Golden Gate entry vector at position 6, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_7'_XcmI-LacZ

Plasmid

#49308

Purpose

Golden Gate entry vector at position 7, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_8'_XcmI-LacZ

Plasmid

#49309

Purpose

Golden Gate entry vector at position 8, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_2'_Linker

Plasmid

#49288

Purpose

Golden Gate entry vector at position 2, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_4'_Linker

Plasmid

#49290

Purpose

Golden Gate entry vector at position 4, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_6'_Linker

Plasmid

#49292

Purpose

Golden Gate entry vector at position 6, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_7'_Linker

Plasmid

#49293

Purpose

Golden Gate entry vector at position 7, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_8'_Linker

Plasmid

#49294

Purpose

Golden Gate entry vector at position 8, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

Plasmid

#195049

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pPGI

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
(F)pGPD(G)_P2 (SBE110)

Plasmid

#194992

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pGPD (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pLDP1

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pFBA

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 450 of 1068 results

Results per page:

Sort results by: