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1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67295

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67290

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

Plasmid

#174883

Purpose

Targeting vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Pou5f1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

Plasmid

#174884

Purpose

Targeting vector for generating Sox2 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Sox2

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174882

Purpose

Targeting vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Nanog

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174881

Purpose

Targeting vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Flnc

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174885

Purpose

Targeting vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Usp5

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174886

Purpose

Targeting vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Wnk1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

Plasmid

#104571

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

human codon optimized ‘dead’ Cpf1 fused to four DmrA domains

Use

CRISPR

Tags

NLS-3xHA-3xFLAG-4xDmrA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTSA-C

Plasmid

#17329

Depositor

Takeshi Sano

Article

Reznik et al Bioconjug Chem. . 12(6):1000-4.

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pRK792

Plasmid

#8830

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-ySet2

Plasmid

#51698

Purpose

Expresses full length yeast Set 2 gene in a mamalian expression vector

Depositor

Tom Misteli

Article

Luco et al Science. 2010 Feb 19;327(5968):996-1000.

Insert

yeast Set2 (SET2 Budding Yeast)

Tags

C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only
C321.ΔA.M9adapted

Bacterial Strain

#98568

Purpose

Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Bacterial Resistance

Ampicillin

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
pUAS-Ncad-GFP

Plasmid

#35986

Depositor

James Jontes, Stephen Smith

Article

Jontes et al J Neurosci. 2004 Oct 13;24(41):9027-34.

Insert

Ncad (cdh2 Zebrafish)

Use

Gal4 inducible

Tags

EGFP

Promoter

14xUAS-E1b

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_TRE_Ncad-P2A-GFPlig

Plasmid

#133806

Purpose

inducible expression of Ncad and membrane-tethered GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

cadherin 2 (CDH2) (Cdh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1- Ncad-GFP

Plasmid

#35676

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Ncad (cdh2 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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