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Plasmid#59123PurposeFAK fret control sensor without FERM domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human Phosphomevalonate kinase (PMK)
Plasmid#52430Purposebacterial expression of human Phosphomevalonate kinase (PMK)DepositorInsertPhosphomevalonate kinase (PMVK Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II no force control (FL-based)
Plasmid#118724PurposeThe no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationtruncation after aa1353PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40
Plasmid#233365PurposeCAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transductionDepositorInsertKinprola_PKA-mEGFP-NLS3X
UseAAVTagsmEGFP-NLS3XExpressionMutationPromoterCAGAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40
Plasmid#233364PurposeCAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transductionDepositorInsertNES-Kinprola_PKA-mTagBFP2
UseAAVTagsNES and mTagBFP2ExpressionMutationPromoterCAGAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40
Plasmid#233368PurposehSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_on-mEGFP
UseAAVTagsNES and mEGFPExpressionMutationPromoterhSyn1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40
Plasmid#233367PurposehSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseAAVTagsNES and mEGFPExpressionMutationPromoterhSyn1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP
Plasmid#233356PurposeCMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_off-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233353PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP
Plasmid#233354PurposeCMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP
Plasmid#233359PurposeCMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP
Plasmid#233360PurposeCMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP
Plasmid#233358PurposeCMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP
Plasmid#233355PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_on-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP
Plasmid#233362PurposeCMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP
Plasmid#233361PurposeCMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD
Plasmid#233363PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubuleDepositorInsertKinprola_PKA-mEGFP-MTBD
UseTagsmEGFP-MTBDExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP
Plasmid#233357PurposeCMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233369PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_PKA-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP
Plasmid#233370PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_on-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only