Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 11,201 results for: NSI;

Showing: 161 - 180 of 11201 results

  2. (+)-Limonene Synthase

    Plasmid
    #112230
    Purpose
    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.
    Depositor
    Daniel Oprian
    Article
    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17
    Insert
    (+)-Limonene Synthase
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-52
    Promoter
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112230-plasmid-map-sequence-id-214310

    Enlarge

  6. pGL3_zfh1_CP-candidate_luc+

    Plasmid
    #86391
    Purpose
    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Promoter
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

    Enlarge

  9. pSTAP-seq_fly-zfh1

    Plasmid
    #86381
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86381-plasmid-map-sequence-id-173935

    Enlarge

  10. pSTAP-seq_fly-tj

    Plasmid
    #86386
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster tj enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86386-plasmid-map-sequence-id-173933

    Enlarge

  11. pSTAP-seq_fly-ssp3

    Plasmid
    #86382
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ssp3 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86382-plasmid-map-sequence-id-173943

    Enlarge

  12. pSTAP-seq_fly-ham

    Plasmid
    #86383
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ham enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86383-plasmid-map-sequence-id-173939

    Enlarge

  13. pSTAP-seq_fly-sgl

    Plasmid
    #86384
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster sgl enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86384-plasmid-map-sequence-id-173940

    Enlarge

  14. pSTAP-seq_fly-ncm

    Plasmid
    #86385
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ncm enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86385-plasmid-map-sequence-id-173945

    Enlarge

  20. eMscL WT

    Plasmid
    #107454
    Purpose
    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107454-plasmid-map-sequence-id-207518

    Enlarge

Showing: 161 - 180 of 11201 results