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Showing: 161 - 180 of 4144 results

  1. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Depositor
    Ronen Zaidel-Bar, Klaus Hahn
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(wt)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus
    Promoter
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53095-plasmid-map-sequence-id-119520

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  3. pCri-H6-TEV-GRAB

    Plasmid
    #128498
    Purpose
    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.
    Depositor
    F Xavier Gomis-Ruth
    Article
    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.
    Insert
    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    Use
    Tags
    His tag (6x)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    128498-plasmid-map-sequence-id-251962

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  5. pOET3-6xHis-hSMSr-ΔSAMD

    Plasmid
    #236225
    Purpose
    Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells
    Depositor
    Chiaki Murakami
    Article
    Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    Hexa histidine tag (6xHis-tag) and enterokinase s…
    Expression
    Insect
    Mutation
    sterile alpha motif domain at N-terminal (1-231 b…
    Promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236225-plasmid-map-sequence-id-471035

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  6. PA-DAD-dark

    Plasmid
    #53096
    Purpose
    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
    Depositor
    Ronen Zaidel-Bar, Klaus Hahn
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(dark)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus; C450M mutation in LOV2
    Promoter
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53096-plasmid-map-sequence-id-119521

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  9. pLV-NT-3-mCherry

    Plasmid
    #190201
    Purpose
    To express the mouse NT-3 tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    NT-3
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190201-plasmid-map-sequence-id-374466

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  10. pLZRS-mVenus-TrkC

    Plasmid
    #182948
    Purpose
    To express the rat TrkC fused in the N-terminus with mVenus
    Depositor
    Pantelis Tsoulfas
    Insert
    mVenus-TrkC (Ntrk3 Rat, Synthetic)
    Use
    Retroviral and Synthetic Biology
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182948-plasmid-map-sequence-id-360203

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  13. pAIP4_5HIH

    Plasmid
    #234205
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234205-plasmid-map-sequence-id-464170

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  14. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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  15. pLZRS-TrkC-mCherry

    Plasmid
    #182949
    Purpose
    To express the rat TrkC fused in the C-terminus with mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    TRk-C-mCherry (Ntrk3 Rat, Synthetic)
    Use
    Retroviral and Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182949-plasmid-map-sequence-id-360115

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  17. pLV-DBNF-mCherry

    Plasmid
    #187195
    Purpose
    To express the mouse BDNF tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF
    Use
    Lentiviral
    Tags
    fluorescent protein mCherry
    Expression
    Mammalian
    Mutation
    The codons have been optimized for better express…
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187195-plasmid-map-sequence-id-374465

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  19. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Insert
    p75NTR-mGFP
    Use
    Tags
    HA tag and mGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

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Showing: 161 - 180 of 4144 results