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Plasmid#208957PurposeA variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)
Plasmid#208958PurposeA variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)
Plasmid#217800PurposeVariant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promotersDepositorInsertPCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
Plasmid#101208PurposeBacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C, N497A, R661A and Q695APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cultivarium POSSUM Toolkit
Plasmid Kit#1000000234PurposeThe POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.DepositorAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (AAV1)
Viral Prep#105321-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (#105321). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7c variant 1513-WPRE plasmid DNA. Synapsin-driven jGCaMP7c expression. jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely-labeled neurons because background fluorescence from inactive neurons is reduced. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (AAV1)
Viral Prep#105322-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (#105322). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7c expression. jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely-labeled neurons because background fluorescence from inactive neurons is reduced. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1F03
Plasmid#71071PurposeGateway entry cloneDepositorInsertSu(var)205 (Su(var)205 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDam-Myc-HP1
Plasmid#59221Purposeexpresses Dam-myc-HP1DepositorInsertHP1 (Su(var)205 Fly)
UseDamidTagsDam and mycExpressionInsectMutationPromoterDrosophila heat shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
KHBD00370
Plasmid#34485DepositorInsertCG8409 (Su(var)205 Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00698
Plasmid#39735DepositorInsertCG8599 (Su(var)3-7 Fly)
UseGateway donr vectorTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00205
Plasmid#34356DepositorInsertCG8068 (Su(var)2-10 Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2F04
Plasmid#70901PurposeGateway entry cloneDepositorInsertmod(mdg4) (mod(mdg4) Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2E12
Plasmid#70940PurposeGateway entry cloneDepositorInsertSu(var)3-3 (Su(var)3-3 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRD60 (dE2F)
Plasmid#14820DepositorInsertE2F (E2f1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
E2F1 in pGEX4t
Plasmid#112976PurposeFor protein expression and purification of Drosophila E2F1DepositorInsertE2F1 (E2f1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP PcCS
Plasmid#139159PurposeSB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana caffeine synthase (PcCS); the other side contains RFP and puromycin resistance genes.DepositorInsertCaffeine Synthase [Paullinia cupana var. sorbilis]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP GST-PcCS
Plasmid#139160PurposeSB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes.DepositorInsertN-GST caffeine Synthase [Paullinia cupana var. sorbilis]
UseTransposonTagsglutathione transferaseExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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pENTR-E2F1
Plasmid#37141DepositorInsertE2F1 (E2f1 Fly)
UseGatewayTagsExpressionMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only