We narrowed to 3,398 results for: guide rna expression plasmid

1. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

   Plasmid

   #211760

   Purpose

   Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

   Depositor

   Glenn Yiu

   Article

   Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, M. mulatta (rhesus macaque), Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPneoHHgRNAH

   Plasmid

   #63557

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPneogRNAH

   Plasmid

   #63556

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. OgeuIscB CTG RNA

   Plasmid

   #222865

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a CTG target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (CTG)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. OgeuIscB GCT RNA

   Plasmid

   #222862

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a GCT target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (GCT)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. OgeuIscB GCA RNA

   Plasmid

   #222864

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a GCA target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (GCA)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. OgeuIscB TGC RNA

   Plasmid

   #222863

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a TGC target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (TGC)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. OgeuIscB AGC RNA

   Plasmid

   #222866

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a AGC target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (AGC)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. OgeuIscB CAG RNA

    Plasmid

    #222867

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a CAG target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (CAG)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Human sgRNA library Brunello in lentiGuide-Puro

    Pooled Library

    #73178

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Mouse sgRNA library Brie in lentiGuide-Puro

    Pooled Library

    #73633

    Purpose

    Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177360

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsine promoter and U6 promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177365

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL6 sgRNA(MS2)-MS2-hA3a D131E

    Plasmid

    #112132

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131E

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131E mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D131E mutant in hA3a

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL5 sgRNA(MS2)-MS2-hA3a D131A

    Plasmid

    #112131

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131A

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131A mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D131A mutant in hA3a

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid

    #52628

    Purpose

    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

    Depositor

    Scot Wolfe

    Insert

    Kanamycin cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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