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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,316 results for: icos

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Showing: 161 - 180 of 13316 results

  2. Human a4 nicotinic subunit crystallization construct

    Plasmid
    #85841
    Purpose
    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85841-plasmid-map-sequence-id-171121

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  3. Human b2 nicotinic subunit crystallization construct

    Plasmid
    #85842
    Purpose
    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    C-terminal StrepII tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85842-plasmid-map-sequence-id-171117

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  5. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

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  6. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid
    #70658
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. Human b4 nicotinic receptor subunit EM construct

    Plasmid
    #163961
    Purpose
    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    StrepII tag and apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Pro341-Ser395 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163961-plasmid-map-sequence-id-323883

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  11. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70659-plasmid-map-sequence-id-162503

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Showing: 161 - 180 of 13316 results