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pDONR223_HSPA5_WT

Plasmid

#82132

Purpose

Gateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSPA5 (HSPA5 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNUT N6His hTFNG

Plasmid

#67240

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

Depositor

Ross MacGillivray, Anne Mason

Article

Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

Insert

human serum transferrin (TF Human)

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

Asn413 Asp, Asn611Asp

Promoter

SV40

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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EasyClone 2.0 Yeast Toolkit

Plasmid Kit

#1000000073

Purpose

An expanded toolkit of integrative vectors for stable gene expression in S. cerevisiae.

Depositor

Irina Borodina

Application

Cloning and Synthetic Biology

Vector Type

Yeast Expression

Cloning Type

USER

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only
pX330A-1x4

Plasmid

#58768

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.1

Plasmid

#172319

Purpose

expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.1 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

Promoter

CMV

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pX330S-6

Plasmid

#58782

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166012

Purpose

mammalian expression of SARS-CoV soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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560HTR

Plasmid

#24444

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PGK1

Plasmid

#23427

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PGK1 (PGK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
plv-AcGFP-SOD1 (wt)

Plasmid

#27138

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

AcGFP

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI

Plasmid

#50723

Purpose

overexpression of human TPI1 in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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plv-AcGFP-SOD1 (G93A)

Plasmid

#27142

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

AcGFP

Mutation

G93A

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pF154 pcDNA3.1(+)SOD1G85R

Plasmid

#26400

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G85R

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only